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SC medium

SC medium contains yeast nitrogen base, ammonium sulfate, a carbon source (usually glucose) and amino acids. Depending on the specific strains and experiments different variations are necessary.

Recipe for 1 liter

  1. 6.8 g Yeast nitrogen base (YNB, including ammonium sulfate, from Formedium)
  2. 0.79 g Complete supplement mixture (CSM, from Formedium)
  3. Fill up to 950 ml and autoclave
  4. Add 50 ml of a 40% glucose solution that was autoclaved separately