NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. In April 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.


SC medium

SC medium contains yeast nitrogen base, ammonium sulfate, a carbon source (usually glucose) and amino acids. Depending on the specific strains and experiments different variations are necessary.

Recipe for 1 liter

  1. 6.8 g Yeast nitrogen base (YNB, including ammonium sulfate, from Formedium)
  2. 0.79 g Complete supplement mixture (CSM, from Formedium)
  3. Fill up to 950 ml and autoclave
  4. Add 50 ml of a 40% glucose solution that was autoclaved separately

Modifications

Remarks